Download this Excel spreadsheet to help scale UHPLC separation conditions to legacy HPLC systems (and vice versa!)

Download this Excel method development spreadsheet to help select conditions for initial gradient scouting runs and to interpret the scouting run results.

• Analytical Training Solutions: A fully on-demand, self-paced learning library covering HPLC, GC, MS, and CE for all levels of experience.
• High-Performance Liquid Chromatography On-Line Course: Shulamit Levin. Also check out the rest of her site; lots of useful information and links about chromatography as well as about chemistry in general.
• Basic Liquid Chromatography: Yuri Kazakevich & Harold McNair. This has been a “work-in-progress” for some time; it is continually being improved and updated. A good, thorough treatment.
• Chromatography: From Dyer Laboratories. This is a brief text-only overview designed to provide background for technical translators.
• Reverse-phase HPLC Basics for LC-MS: from Ionsource. The title says it all.
• Chrom-Ed Series: Chromatography: By R.P.W. Scott. From The Library for Science. Reads very much like a print text converted to pdf format.
• HPLC e-learning from Academy Savant: Not free, but very well done and worthwhile self-paced training modules.

• Chromatography: It’s just a box: Basic definitions and explanations. Especially good for high school students.

Most analytical chemistry journals include liquid chromatography papers. These journals and magazines listed here specialize in chromatography.

• Separation Science: Publishers of on-line and e-mail newsletters on chromatography and related techniques
• LC-GC: This site also has a large number of links to additional chromatography information.
• Journal of Chromatographic Science
• Journal of Chromatography

The US has a number of local/regional discussion groups that focus on chromatography. These groups usually have regular meetings, and their web sites are often extremely valuable reference sources.

• California Separation Science Society (CASSS)
• Central New England Chromatography Council
• Chromatography Forum of the Delaware Valley
• Minnesota Chromatography Forum
• North Jersey Chromatography Group
• Washington Chromatography Discussion Group

You can learn a lot about HPLC just by “lurking”, but don’t be afraid to post questions and, as you get experience, to answer questions posted by others. We’re all in this together!

• Chromatography Forum: We may be just a tad prejudiced (we host this one), but it’s a great site, with great give-and-take from users.

We’re not quite sure how to categorize these, so let’s just say they are repositories of useful information, including even more links:

The LC Troubleshooting Bible: A compendium of John Dolan’s “LC Troubleshooting” columns in LC-GC Magazine from 1983 through 2016 with index and keywords.
HPLC Simulator: a free, online resource for High-Performance Liquid Chromatography (HPLC) simulation, education, and more..
USP Column Equivalency Application: Two databases comparing selectivity of different HPLC columns.
HPLC Columns.org: Selectivity measurements on almost 700 columns from > 30 manufacturers
Some Useful Links for Chromatogaphers: From Douglas D. Frey, Professor, Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County
Mass Spectrometry and Biotechnology Resource: An extensive compilation of information about mass spec.
A “Little” Mass Spectrometry and Sailing:A personal site belonging to James Little with a lot of useful information and links on GC-MS and LC-MS
Chemistry Resources: Heretical as it may seem, there is more to chemistry than just chromatography. This site belongs to Carlos Lopez of Universidad de Antioquia (Columbia) and has a tremendous set of links.