Several variables can be used to change selectivity in a liquid chromatography (LC) separation. Here we compare the variables in an effort to prioritize which experiments will be most effective. LCGC 35(5) 306-311 (2017) John W. Dolan See also 2017-3 and 2017-4 for remaining articles in this series.
Read More →We’ll learn how to find the “sweet spot” in terms of retention for a liquid chromatography separation as well as how much retention change can be expected for a selected change in mobile-phase percent organic or column temperature. LCGC 35(4) 240-245 (2017) John W. Dolan See also 2017-3 and 2017-5 […]
Read More →When considering column efficiency, more is not always better. We look at some ways to quickly estimate the effects of changes in column length and particle diameter rather than trying the experiments in the laboratory. LCGC 35(3) 170-173 (2017) John W. Dolan See also 2017-4 and 2017-5 for remaining articles […]
Read More →Fluorescence detection can be a strong alternative to ultraviolet or other detectors for some compounds. LCGC 34(9) 710-719 (2016) John W. Dolan
Read More →Detectors based on ultraviolet absorbance are the most common detectors in use for liquid chromatography. LCGC 34(8) 534-539 (2016) John W. Dolan
Read More →A reliable autosampler is one key requirement for unattended operatoin of a liquid chromatograph. LCGC 34(7) 472-478 (2016) John W. Dolan
Read More →Different techniques of liquid chromatography (LC) mobile-phase mixing can give different results and have different problems. LCGC 34(6) 400-407 (2016) John W. Dolan
Read More →Understanding how liquid chromatography pumps operate can help streamline solving pump problems. LCGC 34(5) 324-329 (2016) John W. Dolan
Read More →The silica-based packing in reversed-phase columns is not inert. Here we consider what happens when the mobile-phase pH is too high or too low. LCGC 34(2) 106-113 (2016) John W. Dolan
Read More →Strongly retained sample compounds can caus various changes in the appearance of a chromatogram. LCGC 34(1) 20-25 (2016) John W. Dolan Errata: Note that there was a transposition error in this and two associated articles. In the referenced table, the words “Symptom” and “Cause” have been reversed. That is, “Cause” […]
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