2015-4. Be Careful of the Flow Rate

When converting methods from liquid chromatography (LC) to ultrahigh-pressure liquid chromatography (UHPLC), don’t get confused by flow-rate settings. LCGC 33(4) 248-253 (2015) John W. Dolan

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2013-5. Gradient Elution, Part III: Surprises

Sometimes when changes are made to gradient conditions, the result isn’t what was expected. LCGC 31(5) 382-389 (2013) John W. Dolan ERRATUM: The last word on p. 388 should be “decrease” not “increase.” A change in particle size from 3 microns to 5 should reduce the pressure, not increase it.

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2013-3. Gradient Elution, Part I: Intuition

In contrast to isocratic separations, gradient elution sometimes can seem to be counter-intuitive. Yet with the proper perspective, the things we intuitively understand about isocratic methods apply in a similar manner to gradients. LCGC 31(3) 204-209 (2013) John W. Dolan

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