2013-6. Gradient Elution, Part IV: Dwell-Volume Problems

Gradient methods can offer unique solutions to separation problems, but transferring a gradient method from the literature, between laboratories, or even within the same laboratory can be a challenging process. LCGC 31(6) 456-463 (2013) John W. Dolan ERRATA: p. 458, righthand column, 2nd full paragraph, second sentence cites reference (4). […]

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2013-5. Gradient Elution, Part III: Surprises

Sometimes when changes are made to gradient conditions, the result isn’t what was expected. LCGC 31(5) 382-389 (2013) John W. Dolan ERRATUM: The last word on p. 388 should be “decrease” not “increase.” A change in particle size from 3 microns to 5 should reduce the pressure, not increase it.

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2013-3. Gradient Elution, Part I: Intuition

In contrast to isocratic separations, gradient elution sometimes can seem to be counter-intuitive. Yet with the proper perspective, the things we intuitively understand about isocratic methods apply in a similar manner to gradients. LCGC 31(3) 204-209 (2013) John W. Dolan

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