Making LC Methods Work


“Making LC Methods Work” focuses on reversed-phase chromatography, and assumes that you are starting with an existing, validated (or at least, “known to work”) method.

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<strong>Buy additional course registrations now and qualify for a 15% discount.</strong>


Live-On-The-Web: Making LC Methods Work

Who should take this course?

If you use HPLC or UHPLC methods and want a thorough grounding in what to do when problems arise, then , “Making LC Methods work” is the course for you. It’s designed for chemists and biochemists who use LC as a regular part of their jobs, but technicians with some LC experience will also find the course valuable. No previous formal LC training is assumed, but participants should have at least 6 months of “hands-on” experience with liquid chromatography.

What does it cover?

“Making LC Methods Work” focuses on reversed-phase chromatography, and assumes that you are starting with an existing, validated (or at least, “known to work”) method. We’ll cover things like:

  1. Major causes of failure to pass system suitability tests
  2. How to tell whether problems are due to chemistry or hardware
  3. A systematic strategy to identify problem causes efficiently
  4. How to maximize column life
  5. Special techniques such as gradient elution, quantitation, and sample pretreatment
  6. The most effective, timesaving, money saving approaches to preventing common method failures.

. . . And much, much more

How much does it cost ?
The standard “Live on the Web” course fee is $947/person. This includes the course and a download of the course notes. Payment via credit card (MC, VISA, or AMEX only).

Buy additional course registrations now and qualify for a 15% discount. Call us at (925) 297-5374 to arrange multiple-registrant pricing.

What do I need in order to take the course?

  1. A personal computer or tablet (Windows, Mac,iOS,or Android)
  2. A high-speed (broadband) internet connection
  3. A phone connection with headset or speakerphone

Here’s how it works:

  1. After you register we’ll e-mail your course log-in information and password.
  2. Download and print a hard copy of the course handout (approximately 200 pages).
  3. About five minutes before the start of the course, log in to to download the gotomeeting client and check in for the course. Dial the phone number* for the conference call connection that you were given in your confirmation e-mail.
  4. The course runs a total of six hours in three 2.5-hour sessions.

*Standard long-distance phone rates will apply (approximately $10.00).

What if I can’t make it?

All sessions are recorded and will be available for review for a month after the end of the course; if you miss a session, you can catch up at your convenience. If you must cancel, please call at least 48 hours prior to the start of the course in order to receive a refund. No refund will be made for cancellation within 48 hours of the course.

About the instructors
The course was developed by Lloyd Snyder, John Dolan, and Tom Jupille. These sessions will be presented by Tom Jupille.

Registration cost: $947 for a single person. Buy additional course registrations now and qualify for a 15% discount; payment via credit card (MC, VISA, or AMEX only).

The course is taught in three 2.5-hour sessions (Monday, Wednesday, and Friday). Because the course is interactive, the exact timing will vary, but a typical schedule looks like this:

Section 1. How do I know it’s not working?

  • System suitability
  • The troubleshooting process
  • Adjustment vs. modification

Section 2. LC by the numbers

  • Direct measurements on the chromatogram
  • Calculated parameters
  • Retention and selectivity
  • Efficiency
  • Peak symmetry
  • Resolution

Section 3. Gradients

  • When / why use gradients
  • Controlling gradient separations
  • Dwell volume
  • Key differences between gradient and isocratic separations

Section 4. Retention / selectivity issues (reversed-phase)

  • Mobile phase strength
  • Temperature
  • pH
  • Ion pair chromatography
  • Maximizing column lifetime
  • Column substitution
  • Ambiguous documentation

Section 5. Peak width / shape issues

  • Extra-column volume
  • Tubing
  • Fittings
  • Detectors
  • Inlet flow anomalies
  • Stationary phase chemistry
  • Thermal equilibration
  • Overload
  • Late-eluting peaks
  • Section 6. Quantitation
  • Integration
  • Noise and drift
  • Gradient baseline issues
  • Calibration
  • Internal vs. external standardization
  • LOD / LOQ
  • Injector issues

Section 7. HPLC / UHPLC Method Transfer

  • Scaling isocratic separations
  • Scaling gradient separations
  • Possible surprises

Section 8. Diagnostic Tests

  • Pump & detector
  • Gradient proportioning
  • Autosampler

Section 9. Troubleshooting Checklist

  • No peaks
  • Missing peaks
  • Extra peaks
  • Wrong retention time
  • Poor efficiency or peak shape
  • Poor resolution
  • Poor precision
  • Baseline noise or drift
  • Pressure problems
  • Leaks
  • Column lifetime

Additional information

Online Dates

March 20, 22, & 23, Oct. 23, 25, & 27

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