2019-2. Reversed-Phase Liquid Chromatography and Water, Part I: How Much is Too Much?

In reversed-phase separations, retention generally increases as the fraction of water in the eluent increases. When we encounter situations where retention is too low for an analyte of interest, we tend to use eluents with higher and higher levels of water. But how much water is too much?

LCGC 37(2), 80–90 (2019).

Dwight R. Stoll