2014-8. How Much Retention Time Variation is Normal?

Small changes in retention time of a liquid chromatography (LC) method are normal. At what point is a problem suggested? Retention time shifts can be frustrating when you can’t figure out if a shift is something you caused or if there is another reason for it.

LCGC 32(8) 546-551 (2014)

John W. Dolan

Erratum: On p. 548, four lines below the Column Temperature heading, the equation for interconverting temperature in K and C is given incorrectly as K = C – 273. The correct equation is K = C + 273 (or C = K – 273).