The solvent chosen for injection can affect the appearance of chromatograpic peaks.
Bandar Alshli and John W. Dolan
Erratum: (from 30(12): equation 2 should read: w = 4 tR / N^0.5.With this change, all the calculated values of peak widths and recommended injection volumes should be increased fourfold. The discussion and conclusions are still valid. For a fully corrected version, see www.chromatographyonline.com/Dolan1012